Description
ColMA forms stable scaffolds after both thermal crosslinking and photocrosslinking. Human chondrocytes derived from the femoral head were post-seeded on top of ColMA constructs crosslinked with two different methods: thermally at 37 degrees Celsius and through methacrylic group photopolymerization using LAP photoinitiator and exposure to 405-nm wavelength light. Both methods were successful — chondrocytes showed excellent cell viability and homogeneous cell distribution, particularly after seven days of cell culturing. There is a rather minor drop in cell viability for photocrosslinked constructs attributed to the effect of near-UV light. Generally, photocrosslinking is the preferred method for forming ColMA constructs with embedded living cells. ColMA is a unique variation of type I collagen that has been modified with photoactive methacrylate groups, enabling it to be crosslinked in the presence of a photoinitiator. ColMA Solution contains methacrylated collagen dissolved in the acidic Reconstitution Agent A. This biomaterial is a great starting point for your 3D cell culture research, whether you are bioprinting droplets or 3D constructs. Neutralize the solution, with the help of Collagen Buffer, and you are ready to print scaffolds for tissue engineering and high-throughput screening. Recommended for use with BIO X, a temperature-controlled printhead and a heated printbed. Not recommended for use with INKREDIBLE+ or INKREDIBLE. Shipping starts from October 15, 2020
IK4500002001ColMA Solution 5 mg/ml , 20ml
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IK4501022001ColMA Solution 5 mg/ml, LAP, 20ml
KTD225000201ColMA Solution 5 mg/ml Kit, 20ml
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