Laboratory Instruments / Semi-Automated Protein Purification
Laboratory Instruments
The instrument is capable of using 6-well deep well culture plates or 50 mL tubes. In combination with our antibody or recombinant protein binding magnetic beads the system offers high throughput with high capacity. For antibody purification, our newly released AmMag™ protein A magnetic beads allow cleaning of the beads with NaOH to increase the reusability.
For secreted proteins magnetic beads may be added to the cell culture so the binding can occur during the expression. Intracellular proteins can be bound once the cell lysis is accomplished. No centrifugation or filtration are necessary saving you hours of pre-processing time.
AmMag SA is semi-automated magbeads purification device for antibody/protein discovery and development.
Faster：Complete 12 samples within 40 min
Low endotoxin：UV + closed working station
Automatic washing and elution process
Comparable with 50 ml tubes and 6-well plate
Eliminate the need for centrifugation and filtration
AmMag™ SA Semi-automated System
Purifies up to 12 samples at a time
Sample volumes from 5 ml to 50 ml
Up to 80 mg of protein or antibody per sample
Typical cycle time for 12 samples is less than 40 minutes
AmMag™ SA Semi-automated System
1
Use with larger samples (few liters)
The AmMag™ SA contains adapters for entering samples in 15 mL and 50 mL tubes. However, larger samples can be easily purified using the AmMag™ SA by simply collecting the magnetic beads from these samples after the binding step, and transferring the beads into 50 ml or 15 ml tubes. To help collect magnetic beads from larger samples, we provide the AmMag™ Wand and the AmMag™ Block.
2
Closed environment to minimize endotoxin contamination
The AmMag™ SA is equipped with air filters to clean incoming air, and further maintains a positive air flow through the instrument. These features prevent airborne endotoxins from entering the system and contaminating samples. To clean the instrument between runs, all tubing can be flushed and incubated for up to an hour with 0.1 N NaOH.
3
Complete washing and elution process automatically
After cultures have expressed the desired proteins or antibodies, magnetic beads are added to bind the targets. After this step, the cultures are transferred to 50 mL tubes and then moved to AmMag™ SA. The automated washing and elution steps purify the target proteins and antibodies from the magnetic beads.
4
Eliminate the need for centrifugation and filtration
The AmMag™ SA utilizes magnetic beads for purification. Since magnetic beads are free-flowing, cells and debris from cultures do not prevent them from binding target protein and antibodies. This eliminates the need to clear cultures by centrifugation and filtration prior to binding with magnetic beads.
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