Laboratory Instruments / Logos Biosystems Automated Cell Counter
Laboratory Instruments
As is well-known in brewing and winemaking industries, the quantity of yeast pitched to initiate fermentation directly affects the flavor, aroma, and quality of the final product. Although yeast can be pitched by weight or volume, such methods are inexact and highly variable. The ASBC method using methylene blue method is more accurate but time consuming and subjective. Use the LUNA-II YF™ to get accurate and reproducible yeast cell counts in as little as 15 seconds. This little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow
Work smarter, not harder
Details like never before
UV-crosslinking system
The UV-crosslinking system hardens the structure, allowing it to be moved without losing shape. Both the INKREDIBLE and the INKREDIBLE+ are delivered with 365 nm LEDs, and the INKREDIBLE+ has a 405 nm option to extend the range of crosslinkable bioinks.
Dual printheads
The dual printheads allow you to use different cell types in the same structure without switching cartridges or pausing the bioprinting process. This makes it possible to bioprint with scaffolds enables the INKREDIBLE to produce complex structures that would otherwise collapse.
Clean Chamber Technology
CELLINK’s patented Clean Chamber Technology enables the INKREDIBLE+ to operate outside of a biological hood – creating a true desktop 3D bioprinter! With HEPA-filtered positive air pressure inside the printing chamber, you can be sure that your bioprinting is sterile.
Results in five steps
Software
AMR-100 keeps with high visual and logical user interface of software, it can be offered a comprehensive inbuilt
calculations, such as blank subtraction, quantitative curve fit, quanlitative classification and kinetic calculations,
as well as the versatile reporting tool and make data reduction.
Filters
The AMR-100 Microplate Reader comes with four standard absorbance filters:405, 450, 492, and 630nm. We
also supply custom filters at 5~9nm intervals from 340 to 750nm on request.
New fluorescence detection function
Fluorescence detection combined with fluorescence quantitative analysis kit, able to accurately quantify DNA, RNA and protein concentration through the specific binding of fluorochrome with target material, and the minimum limit is 0.5pg/u(dsDNA).
Nano-500 can be compatible with common fluorescence quantitative reagent to provide users with maximum convenience and minimum detection cost.
Technical specification
|
Model |
MS-3000 |
|
Display |
4.3 inch touch screen (capacitive type) |
|
Temperature control range |
RT +5℃~105℃ |
|
Temperature uniformity |
≤0.5℃ |
|
Timer range |
1min~99h59min |
|
Temperature control accuracy |
±0.5℃(@30-50℃) |
|
Display accuracy |
±0.1℃ |
|
Shaking speed |
200-3000rpm |
|
Heating time (25-100℃) |
≤15min (RT 20℃～30℃) |
|
Refrigeration time(detected at 25℃ ambient temperature) |
—— |
|
Heating rate gear |
Max; 3℃/min; 2°C/min;1℃/min; 0.1℃/min |
|
Cooling rate gear |
Max;1℃/min;0.5℃/min;0.1℃/min |
|
Program number |
50 |
|
External port |
USB: program related, printer; 7Pin socket: thermo lid |
|
Shaking amplitude |
3mm |
|
Dimension(mm) |
318x154x220 |
|
Weight |
10kg |
Technical Specifications
| Model | MB100-4A | MK100-4A |
| Temperature Control Range | RT.+5°C~70°C | RT+5°C~70°C |
| Temperature Setting Range | 5°C~70°C | 5°C~70°C |
| Timer | 1min~99h59min | 1min~99h59min |
| Temp. Control Accuracy | ±0.5°C | ±0.5°C |
| Display Accuracy | ±0.1°C | ±0.1°C |
| Shaking Speed | 100~1500rpm | ---- |
| Heating Time(20 to 70°C) | ≤20min | ≤20min |
| Platform Capacity | 4 plates | 4 plates |
| Mixing Orbit | 2mm | ---- |
| Dimension(mm) | 320 x 350 x 185 | 320 x 350 x 185 |
| Net Weight | 9kg | 8kg |
Wireless BIO X6
Develop your most complex constructs without the need for external connections, detach the tablet to take it with you wherever you go. Simply reattach it when you’re ready to print again.
Wired BIO X6
The tablet’s surface makes it easy to enter your parameters with the swipe of a finger, even when your gloves are on.
Integrated Ethernet port:
Leverage the built-in Ethernet capability with any standard Ethernet cable.
Fast file and data transfer:
Connect the tablet directly to your computer to transfer your models in seconds.
FOR ALL APPLICATIONS
The BIO X6 is a versatile bioprinter equipped with intelligent printhead mounts, enabling you to easily upgrade your system as we develop printheads to meet your evolving needs.
– Heated Pneumatic Head (65° C).
– Thermoplastic Head (250°).
– Temperature-controlled Pneumatic Head (4-65°).
– Electromagnetic Droplet (EMD) Printhead (65°).
– Syringe Pump Printhead.
– Photo-curing Tool Head, for UV light in any wavelength.
– HD Camera Tool Head.
Clean, reinvented
With dual high-power fans producing a powerful airflow through its dual-filtration top, BIO X bioprinter creates a positive pressure inside the chamber. The HEPA H14 dual-filter top retains more than 99.995% of unwanted particles and microorganisms. BIO X is equipped with UV-C germicidal lamps that allow you to run automatic cycles to sterilize the printing environment.
Printheads
BIO X is the first 3D bioprinter in the world with Intelligent Printheads (iPH), ensuring your research is always on the cutting edge. BIO X printheads are designed with flexibility, beauty and simplicity in mind. The best part is that you can design your own dispensing technologies or methods to use with BIO X system. The possibilities are endless, giving you full freedom in your lab work.
Wide material range
BIO X allows you to delicately control the temperature of its print bed, enabling a new level of printing quality. Being able to control the print bed temperature paves the way for using any bioink, no matter the viscosity.
15 Seconds to Target
The automated cell counter LUNA II™ has integrated a novel focussing mechanism based on liquid lenses technology.
The liquid lenses do not use any mechanical moving parts to change the Z stage.
Instead, the position of the sample is rapidly obtained by the application of a small voltage to the liquid lens. The elimination of moving parts removes noise and significantly reduces the need of servicing. Even after multi-million cycles of focussing, the liquid lens does not require repair or service.
In combination with this novel focussing mechanism, the LUNA II™ has integrated a new autofocusing algorithm optimized for cell counting
01 HYDROGEL INFUSION
The X-CLARITY™ Hydrogel Solution Kit is a pre-tested hydrogel solution for uniform and consistent tissue-hydrogel hybridization. The kit is made up of X-CLARITY™ Hydrogel Solution, an acrylamide-based solution used to create polyacrylamide, and X-CLARITY™ Polymerization Initiator, a thermal free radical initiator also known as VA-044
Under control
The LUNA-II™ can precisely and consistently distinguish live and dead cells from debris.
02 TISSUE-HYDROGEL HYBRIDIZATION
The X-CLARITY™ Polymerization System is a standalone, automated system developed to simplify tissue-hydrogel hybridization, a crucial step for optimal tissue clearing. Multiple samples can be placed in multi-well plates or conical tubes for rapid and efficient high-throughput sample processing. Users can control polymerization by adjusting vacuum strength, temperature, and a timer through a simple touchscreen interface.
SOFTWARE
The LUNA II™ cell counting algorithm has been recognized to have the best-in-class cell counting accuracy. The LUNA II™ software has exceptional accuracy in cell de-clustering and can successfully count clumpy cells. The LUNA II™ automated cell counter has inherited this well-known performance aspect of the LUNA™ software.
Clumpy cells are de-clustered quickly, as well as automatically counted as individual cells
03 LIPID REMOVAL
The X-CLARITY™ Tissue Clearing System II is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. Clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology. Users can set tissue clearing conditions through a simple and intuitive touchscreen interface. In ETC (electrophoretic tissue clearing) mode, platinum-plated electrodes generate an electric field to accelerate the removal of lipids from tissues in a highly efficient manner. A built-in temperature control system actively cools and heats buffer to maintain consistent buffer temperatures during clearing. Buffer is constantly circulated to ensure consistent buffering capacity, temperature control, and elimination of tissue clearing byproducts. This advanced system ensures efficient, rapid, and consistent tissue clearing.
REPORTS
Print data with the built-in printer or export high-resolution images, data,
and full data reports via USB.
THE ALL-IN-ONE SYSTEM FOR EXCEPTIONAL IMAGING QUALITY
Live cells can be imaged in time lapse mode in a precisely controlled environment. The images can be used to create a video with annotations.
REUSABLE SLIDES
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
HIGH EFFICENCY OPTICAL DESIGN
Advanced precision optics and a highly sensitive camera allow you to capture high resolution, multi-channel fluorescence images.
ALL WHAT YOU NEED
Choose from a variety of tools to analyze and annotate your images. Measurement data can be saved onboard or to a USB drive.
ALL IN FOCUS
Capture images of multiple focal planes in Z-stack mode. Images can be focus stacked to produce one image or combined into a video.
MEASURING SPEED AND ACCURACY
Capture, analysis and measurement of 3 fluorescent images in just 30 seconds and in bright field mode you will get one image and all measurement data in just 10 seconds.
CUSTOMIZE
· Create and customize image analysis projects
· Quantitatively analyze multiple image-based phenotypes
AUTOMATIC COUNTING AND ANALYSIS
Check cell concentration and viability in cell counter mode. The CELENA® S automatically adjusts light and focus for optimal results.
MICROSCOPY LIKE HIGH RESOLUTION
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.the capture and analysis process takes just a few seconds
FROM 0 TO 96 WELLS....... DURING COFFEE BREAKS
Automated vessel handling and scanning
Motorized XYZ stage, filter cube stage, and objective turret
LED FLUORESCENCE AND DEDICATED OBJECTIVES
12+ hard-coated LED fluorescence filters Infinity-corrected objectives with a magnification range of 1.25-100X
Dual Fluorescence
The Luna-FL™ automated cell counter from Logos Biosystems is a quantum leap for automated cell counting and cell viability analysis.
The Luna-FL™gives you sensitive and accurate live/dead cell counting results without limitations of cell types.
Unlike immortalized cell lines primary cells such as PBMCs splenocytes, neutrophils, and stem cells have been difficult to count with conventional cell counters such as coulter counter or image-based automated cell counters that utilize bright field optics.
Primary cells are often contaminated with undesirable debris, which can be confused with cells with conventional cell counters.
The Luna-FL™ is integrated with dual fluorescence microscope optics to overcome this problem.
Live/dead cells are stained with green/red fluorescence dye, and labelled cell images are analysed with accurate image analysis software
GET AUTOFOCUSSED
· Rapid and reproducible focusing
· Minimized phototoxicity and photobleaching
Data report
The analysed data can be easily saved as a PDF or CSV file. Cell counting results, cell image data, and various type of histograms are integrated into a single PDF file and transferred via USB drive. Data from previous cell counts (up to 1000 counts) are archived in the LUNA-FL™
EASY TO WORK
Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging
COST EFFICENCY
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
USER AT THE CENTRE
· Simple setup of imaging protocols
· Seamless integration of imaging and data analysis processes
ALL WHAT YOU NEED WITHIN REACH
Onstage incubation system for a variety of experiments in physiological and non-physiological conditions
THE LOWEST COST. THE GREATEST CONVENIENCE.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
SPECIFIC AND OPTIMIZED ANALYSIS
We’ve independently tested 30+ brewing yeast products on the LUNA-II YF™.
SPECIFIC AND OPTIMIZED
Unlike flow cytometers, the LUNA-II YF™ has advanced declustering
capabilities, and can count even the clumpiest cells.
SPEED AND ACCURACY
Using dual fluorescence optics, an autofocusing liquid lens, and advance
software, the LUNA-II YF™ produces data in just 15 seconds.
WHAT THE LUNA-II YF™ DOES BEST
The LUNA-II YF™ provides cell count and viability data with the precision
and consistency of a flow cytometer.
REPORTS
PDF reports complete with images and data are automatically generated and can be transferred to a PC or printed for your records.
WHAT THE LUNA-STEM™ DOES BEST
Nucleic stains make sure that nucleated cells are counted as live or dead cells whereas nonnucleated cells are counted as non-nucleated cells.
30 SECONDS FASTER
In as little as 30 seconds, the LOGOS LUNA-STEM™ captures, merges, and analyzes images from three channels to produce cell count and viability data.
CANNOT BE WRONG
Using dual fluorescence and brightfield optics, the LUNA-STEM™ can distinguish between cells and debris.
LA MIGLIORE PRECISIONE
L'algoritmo di calcolo avanzato e l'aumento dei volumi di misura riducono il CV, anche a meno dell'1%!
PIÙ CAMPIONI IN UNA SOLA VOLTA
I nuovi vetrini a 8 e 3 camere aumentano la produttività.
GAMMA DI CONCENTRAZIONE CELLULARE AMPLIATA (1.0E04 - 5.0E07 CELLE/ML)
Adatto per diverse applicazioni di conteggio delle cellule, tra cui il sequenziamento di singole cellule e le determinazioni di dosaggio CAR-T.
ECCELLENZA NEL CONTEGGIO SIA IN CAMPO CHIARO CHE IN FLUORESCENZA
L'autofocus rapido e preciso l’ algoritmo software ottimizzato, sono progettati per lavorare in tutte le modalità di conta desiderate.
SICUREZZA, FIDUCIA E AFFIDABILITÀ
Il software integrato e le slide di validazione per campo chiaro e fluorescenza garantiscono il monitoraggio e la reportistica giornaliera.
CONFORMITÀ 21 CFR PARTI 11
Il software CountWire™ fornisce la gestione integrata della sicurezza dei dati e dell'accessibilità.
LA FINE DEL DIBATTITO SULL'ACCURATEZZA DI MISURA
Il conteggio manuale e la maggior parte dei contacellule automatizzati rilevano le cellule in un volume di conteggio di ~ 0,5 μl. Il LUNA-FX7™ utilizza un volume di conteggio fino a 5,1 μl, riducendo l'errore e il CV per ogni conteggio. Il miglioramento degli algoritmi di de-clustering e l'autofocus preciso aumentano, ulteriormente la precisione e l'affidabilità del LUNA-FX7™.
FACILE, FLESSIBILE E ROBUSTO
Il funzionamento semplice ed efficiente tramite comandi touchscreen intuitivi, le opzioni di rilevamento a campo chiaro e doppia fluorescenza, le modalità di conteggio multiple e i protocolli personalizzabili, consentono di ottimizzare facilmente la vostra cellula e il tipo di campione. I risultati del conteggio possono essere esportati come un riepilogo CSV o un report completo in PDF con immagini in miniatura e taggate. Con un disco rigido interno da 250 GB, non è necessario un computer aggiuntivo e i dati possono essere esportati via Wi-Fi, Ethernet o chiavetta USB.
NUOVA MODALITÀ DI BIO-PROCESSAZIONE
È possibile produrre tassi di crescita e grafici di tendenza che consentono di tracciare la produzione e di prevedere con precisione le tempistiche.
FIDATEVI DEI VOSTRI DATI
I vetrini di convalida in campo chiaro e fluorescenza offrono un'esatta concentrazione e vitalità delle cellule, mentre le funzioni del software offrono la possibilità di memorizzare, monitorare e analizzare i dati
PRODUTTIVITÀ, CONTROLLO
PRODUTTIVITÀ, CONTROLLO ED EFFICIENZA
L'intuitiva interfaccia utente consente di passare facilmente da un formato all'altro e di designare esattamente il canale da analizzare consentendo l'utilizzo futuro di canali non ancora utilizzati. Con l'inserimento delle slide "One and Done", una slide deve essere inserita una sola volta nel contatore, senza doverla rimuovere e reinserirla per analizzare diversi canali.
CONFORMITÀ 21 CFR PART 11 (IN ARRIVO)
Il nostro innovativo software CountWire™ consente di accedere e gestire in remoto i dati di più unità LUNA-FX7™ collegate alla rete.
Andile Mtshali
CAPRISA
It's more convenient, saves time. Very effective, minimizes errors possed by manual counts.
Michael Schnekenburger
LBMCC
The device is user friendly, provide relatively fast and very accurate results. It works for all kind of cell types, adherent and suspension cell lines, CD34, PBMCs... The algorithm is doing a fantastic job to discriminate healthy from dead cells and it is amazing how it can accurately count cells even in cell aggregates. Pretty good interface to see, manage and export your data (including Wifi connection).
Karan Singh
NYU Langone Medical Center
It is excellent because of the following: 1-Its ease of use. 2- Reproducibility of data. 3- Take less time to process than others. 4- Reliable. 5- Value for the money.
Emma Rogers OBT sciences
OBT sciences
The LUNA FX7 Automated Cell Counter has streamlined our workflow and increased the repeatability of our internal experiments. This equipment allows multiple users to achieve verifiable outputs and has increased our capacity at a complicated time when our personnel are limited by their in-person activities. The fluorescence and imaging capabilities of this cell counter has reduced our reliance on older, more specialized equipment. The company was responsive, engaged, and knowledgeable about the right product for our uses.
Katja Salo
University of Helsinki
Device has been easy to use, and simple for a new user. Autofocus works nicely which is important to us; we need consistent results because different people run cell counting as a first step of single cell analysis. Algorithm seems to work accurately as it differentiates between actual cells and cell debris or other particles. Counting with the 8-chamber slide is quite consistent, you just have to pay attention to even pipetting. Measuring with the 8-chamber slide is super easy and quick compared to Luna-stem that we used to use. Getting 8 counts and also saving all the files with two keystrokes frees you to do something else while the counter is running.
Giorgia Montalbano, Giulia Molino, Sonia Fiorilli, and Chiara Vitale-Brovarone
University of Turin
Challenge
Osteoporosis and degradation of bone mechanics affects millions of people each year, often leading to fractures and immobility. Therefore, regenerative medicine strategies are urgently needed. Bioprinting complex geometries with collagen, however, can be challenging and most scaffolds based on type I collagen and hydroxyapatite are fabricated by freeze-drying or casting, application that give bioengineers limited control over the final shape and porosity of their constructs.
Solution
The team at the Polytechnic University of Turin mitigated these challenges by combining the BIO X, a temperature controlled printhead and gelatin-supporting baths (the FRESH method) to 3D bioprint taller, more complex bone scaffolds with type I collagen.
Results
The study validates the potential for collagen nanocomposites as a material for high-resolution 3D bioprinting. The authors also suggest that using the hybrid material represents a significant step toward fabricating biomimetic patient-specific bone-like scaffolds on demand.
Learn more
WATCH THE VIDEO HERE!
Synthesis and incorporation of rod-like nano-hydroxyapatite into type I collagen matrix: A hybrid formulation for 3D printing of bone scaffolds. Journal of the European Ceramic Society. 2020. DOI: 10.1016/j.jeurceramsoc.2020.02.018.
Silvia Pisani, Rossella Dorati, Franca Scocozza, et al
University of Pavia
Challenge
Typically, gelatin, collagen, alginate and fibrin hydrogels are used in 3D bioprinting and tissue engineering because of their extracellular matrix (ECM) properties. However, the search for other natural ECM-like polymers has lagged behind. Chitosan, a natural, biocompatible and biodegradable polymer, has been approved by the FDA and EMA for use in wound dressings, but it is not stable under physiological conditions.
Solution
Conti et al. discovered that because of their ionic interactions and rheological properties, 3D bioprinting of chitosan and gamma-PGA structures together at certain concentrations of the biopolymer and crosslinker resulted in more favorable constructs. The CELLINK INKREDIBLE+ bioprinter was used to explore the bioprinting properties of this bioink formulation in order to compare it to other natural ECM material formulations.
Results
The 3D structures generated by the INKREDIBLE+ confirmed that the chitosan-gamma-PGA formulation is biocompatible and stable under shear stresses greater than that of human arteries and veins.
Read more
Preliminary investigation on a new natural based poly(gamma‐glutamic acid)/chitosan bioink. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2020. DOI: 10.1002/jbm.b.34602.
Tracey Geneau
Greenfield Ethanol
I'm testing the LUNA-II and it works great. I work for an ethanol plant and our operators count yeast. This will be a great way to streamline the yeast count results. It will also please our operators.
Paul Kim, PhD
Grambling State University
This is a nice automated cell counter that is well worth considering. We count cells to set up experiments with a consistent number of input cells and to express the resulting data accurately. An automated cell counter makes this a quick, painless, reproducible process. Tip: get the one with the printer!
Clémence Jacquemin
PhD INSERM
Rapid, simple, reliable. This product is easy to use. The fact that one can use a reusable blade is an advantage in terms of cost when one line is to be counted. The results are reliable, we have compared them to those obtained in Molasses cells, against the concentration must be close to 1x10 ^ 6 cells / mL. I am very satisfied with this purchase, which is unanimous within the team.
Dale Taylor
University of Melbourne
Fantastic easy to use machine
The LUNA-II is a fantastic product that has allowed me to create reproducible experiments within my cell culture. The instrument is extremely easy to use with a user friendly interface as well as appropriate complementary instructions online. The machine has never had any issues or faults after over a year of heavy use and as such I have recommended it to other labs with heavy tissue culture usage!
Joanna Ireland, PhD
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
May Malicdan, MD PhD
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased
Woong Sun, PhD
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
Regina Wulff, MS
Weill Cornell Medicine / New York-Presbyterian Hospital
The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
Mikayla Lopes
Roger Williams Medical Center
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!
Gelina Sani
Children's National Medical Center
The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.
Myoung Sup Shim, PhD
Duke University Health System
The best automatic digital imaging system
We need live cell time-lapse imaging for our studies but other imaging systems did not produce acceptable images for long-term imaging. The CELENA X is the only system that has given us satisfactory multi-fluorescence images of multi-well plates. The automatic laser focusing is especially fantastic. We don’t waste time taking individual images as the CELENA X does high-throughput imaging and focuses great for all wells over long periods of time. Image quality is great for every photo and making high-quality videos is easy. Cells remain healthy, and there is minimal fluorescence quenching even after extensive imaging (5 minute intervals for over 72 hours). I strongly recommend the CELENA X for live cell time-lapse imaging and other applications.
LUNA-II YF™
1
Explore new dynamics
5X increased sensitivity for secreted analytes in supernatants
Upto 7 custom timed supernatant samples can be collected per sample
>95% fluid transfer reproducibility during assays
2
Streamline your co-cultures
Seamless immune cell transfers with no loss of viability
Only 15-minute flowchip prep time &
5-minute protocol set up
Only 1-Touch to run your assay protocol for hands-off workflow
3
Improve your IF Staining
Zero organoid perturbation during multiple assay steps
Reduce pipetting by 20X - Save 5 hours of hands-on time
10X more precise organoid location for microscopy
4
Designed to operate directly inside your incubator
HoloMonitor M4 is designed to operate 24/7, directly inside your normal incubator. This gives you the possibility to continuously record your cells in their natural environment, making sure you don't miss any important cellular events in your culture.
5
Non-invasive — no labels or toxic stains needed
HoloMonitor M4 is based on digital holography, meaning that the cells in your culture are imaged with nothing but light. The technique doesn't require any additives to the cell culture medium, nor does it require antibodies or stains to visualize your cells. The images are built from the low-energy light beam, alone.
6
Gives quantitative data on single-cell level
The HoloMonitor App Suite software will reconstruct your cells in 3D, immediately providing you with quantitative information about each cell. More than 30 different morphological parameters are calculated automatically, such as cell area, thickness and volume. Of course, all data can be compiled, exported and analyzed at population level as well.
7
Powerful and unique application portfolio
The HoloMonitor App Suite software offers a wide range of applications, each designed to allow for easy setup and analysis of a specific kind of experiment. We offer both end-point oriented applications, such as an automatic cell counter, and kinetic applications, such as cell tracking and dose-response assays.
8
Convenient and easy to use
HoloMonitor and the App Suite software is developed with user-friendliness in mind. Just place your cell culture vessel onto the microscope and let the intuitive software guide you through the experimental setup. When finished, relevant results are generated automatically. Naturally, you can export all underlying data to make your own analysis as well.
9
Saves time, money and cells
The HoloMonitor system allows for quick and guided experimental setup, runs the imaging completely automatic, and doesn't require any reagents to run. Furthermore, you can reanalyze one experiment in multiple ways, generating several results from fewer cells. Taken together, these aspects save you time, money and cells.
10
Completely Hands-off
Load your reagents and Go
11
Programmable
Easily customization program for protocol need
12
Antibody Saving
Automatically recover primary antibody for re-use
13
Consistent Results
Fully automated operation ensuring consistency every time
14
Use with larger samples (few liters)
The AmMag™ SA contains adapters for entering samples in 15 mL and 50 mL tubes. However, larger samples can be easily purified using the AmMag™ SA by simply collecting the magnetic beads from these samples after the binding step, and transferring the beads into 50 ml or 15 ml tubes. To help collect magnetic beads from larger samples, we provide the AmMag™ Wand and the AmMag™ Block.
15
Closed environment to minimize endotoxin contamination
The AmMag™ SA is equipped with air filters to clean incoming air, and further maintains a positive air flow through the instrument. These features prevent airborne endotoxins from entering the system and contaminating samples. To clean the instrument between runs, all tubing can be flushed and incubated for up to an hour with 0.1 N NaOH.
16
Complete washing and elution process automatically
After cultures have expressed the desired proteins or antibodies, magnetic beads are added to bind the targets. After this step, the cultures are transferred to 50 mL tubes and then moved to AmMag™ SA. The automated washing and elution steps purify the target proteins and antibodies from the magnetic beads.
17
Eliminate the need for centrifugation and filtration
The AmMag™ SA utilizes magnetic beads for purification. Since magnetic beads are free-flowing, cells and debris from cultures do not prevent them from binding target protein and antibodies. This eliminates the need to clear cultures by centrifugation and filtration prior to binding with magnetic beads.
18
Flexibility
Our customizable protocols allows optimal flexibility for your research. The unit comes with standard protocols designed to transfer larger proteins (up to 400 kD) or opt for a shorter transfer time for smaller size proteins (up to 100 kD).
19
Convenience
eBlot™ L1 allows fast and efficient transfer of 1 or 2 mini gels at a time. The setup takes less than 2 minutes and avoids the mess of a traditional wet transfer with our easy to assemble dry sponge sandwich.
20
Benefits of eBlot™ L1
Comprehensive: Outstanding performance for small, medium and large proteins
Convenience: Simultaneously transfer two mini gels
Compatible: Highly compatible with different types of precast or homemade gels
Simple: Easy to assemble, one button operation and customizable programs available
21
Why choose eBlot™
Conventional wet transfers are cumbersome, require experience and a great deal of time spent on consumable preparation. The transfer times usually vary from 1 hour to overnight. With eBlot™ fast wet transfer system, achieve excellent transfer efficiency without sacrificing speed.
22
• Disposable single use chips and contained waste minimize risk of cross contamination between measurements and reduce cleaning and setup times
23
Easy-to-use interface and consumable management
24
High reproducibility and accuracy (even at low concentrations)
25
Low sample requirement — Just 5μl per sample
26
In-solution, binding affinity measurement using microfluidic diffusional sizing (MDS)
27
Disposable single use chips and contained waste minimize risk of cross contamination
28
Easy-to-use interface and consumable management
29
Independent determination of antibody concentration and affinity for a comprehensive immune response assessment
30
No binding artifacts or non-specific binding, no surface-constraints
31
Ability to measure in complex backgrounds such as minimally diluted serum
32
Characterization of protein interactions in solution using microfluidic diffusional sizing (MDS)
33
High purity and excellent yield of nucleic acid
34
Very simple operation (easy to install, operate, maintain) PC not needed
35
Very fast extraction protocol, 15~40 minutes / cycle depending on sample type and method
36
Auto-Pure96 can process 96x1ml samples simultaneousl
37
Accurate temperature control system ensures stability of?purification performance
38
High-throughput:
39
Patent structure design with center rotary table which guarantees stability and liability (Success rate >99.99%)
40
Touchscreen interface and easy operation, parameters can be tuned based on experimental requirements
41
Yeast specific application software
42
Disposable Slide option to reduce possible contamination
43
Reusable slides to cut costs
44
Quick and easy plug and play system
45
Stand-alone system with easy-to-use 7.0” LCD touch screen and software
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Measurement of single wavelength as well as spectrum analysis using spectrometer technology
47
Modes: DNA/RNA, protein, cells, end point, kinetic, spectrum
48
Simple data backup using USB
49
Upload of custom made protocols
50
Extraordinary tissue transparency for superior imaging quality
51
Quick and easy implementation
52
Faster processing times
53
Artifacts minimized
54
Best price / performance ratio
55
No tissue damage
56
Immersion lenses for higher resolution and autofocus
57
Reusable slides to cut costs
58
Disposable slide option to reduce possible contamination
59
Built-in printer or export of reports and high resolution images to USB media
60
Quick and easy plug and play system
61
Outstanding price/performance ratio
62
Customizable for your cell imaging needs
63
Simple and comprehensive image analysis software
64
The absence of eyepieces protects different operators from possible viruses transmission
65
Compact and vibration-free design
66
Fully automatic imaging system
67
Automatic well scan
68
Simple and comprehensive image analysis software
69
The absence of eyepieces protects operators in possible viruses transmission
70
Compact and vibration-free design
71
ALL INCLUDED
Turnkey solution: Hardware Software and consumables compatibility problems are just a memory
72
Laser autofocus for precise and fast focusing
73
COMPACT FOOTPRINT
BOTH COMPRESSOR AND COOLING UNIT HAVE BEEN INTEGRATED WITHOUT COMPROMISING ITS SIZE
74
CLEAN CHAMBER FOR BETTER CELL VIABILITY
Built-in UV sterilization and a HEPA filter bring the sterility of the biosafety cabinet to the benchtop.
75
TARGET ON SIGHT
Reduction of failures and faster clinical trials
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Six printheads
Six printheads for scaffolds and complex vascular networks
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Everything sterile
Total sterilization for superior security
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Target on sight
Reduction of failures and faster clinical trials
79
Speed
Quick and easy to reduce learning time
80
Your Advantages
1. Easy to use with 7 inch touch screen together with 3 external keys
2. A wide wavelength range from 340 to 750 nm
3. Able to operate autonomously or connect via PC and export the results to an Android device
4. Absorption range: 0.0~4,000Abs
5. 8-position filter holder disc, equipped with 4 standard optical filters
6. Fast and precise measurement of 96-well plates in 6 seconds
7. Convenient built-in stirrer
8. Built-in large format thermal printer
9. Endpoint, kinetic and multi-label measurements for various applications
10. Agitator with variable time and speed
81
Your Advantages
1. Automatic detection
2. Android system, 7-inch capacitive touch screen, intuitive APP software, simple and easy to use
3. New OD600 function desiged to ease bacteria and microorganism concentration detection
4. Detection and display in 6 seconds
5. HD touch screen, no need to connect computer.
6. Built-in printer and USB for data analysis storage and export
82
Your advantages
1. LCD display. It is easy to set up and use
2. Heating plates above and below the microplates
3. Incubation up to 70°C in 0.1°C steps
4. Shakes and incubates two or four microplates simultaneously
5. Beep-signal and stop of shaking motion after program completion
6. Excellent temperature accuracy and uniformity across the plate
7. Exact correspondence of the designed and actual temperature
8. MB series have incubation and shaking functions
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All included
Turnkey solution: Hardware Software and consumables compatibility problems are just a memory
84
ALL INCLUDED
Turnkey solution: Hardware Software and consumables compatibility problems are just a memory
85
Best Price/Performance ratio for fluorescence based systems
86
Easy and fast Plug and play
87
Reusable Slides as perfect labs on a budget solution
88
Disposable Slide option to reduce possible contaminations
89
Dual fluorescence GFP e RFP
90
Best price / performance ratio for fluorescent systems
91
Opzioni per vetrini fino a 8 canali per una altissima produttività
92
Volume per il conteggio fino a 5.1 μl, riducendo l’errore di misura e CV per ogni conta
93
Ampliamento della concentrazione cellulare (1.0E04 - 5.0E07 cellule/ML) Per sequenziamento di singole cellule e le determinazioni di dosaggio CAR-T.
94
Autofocus per immagini in campo chiaro e fluorescenza
95
Conforme alla normativa 21 CFR Par11 per la gestione del lavoro in rete in totale sicurezza
