Molecolar and cell biology / Consumables and accessories for instruments

Standard Bioinks

Molecolar and cell biology

Discover more

Standard Bioink

GelMA 20%

GelMA 20% has the same composition as the former product GelMA High.

GelMA is porcine gelatin functionalized with methacrylate groups. GelMA 20% is a concentrated solution at physiological pH and osmolarity, to be used as a base in bioink formulation. Dilute the GelMA solution with our Reconstitution Agent P, or mix with other additives, and use in 3D cell culture. Mixed with a photoinitiator, GelMA hydrogels are easily photocrosslinked to become stable during cell culture. Preparing your own GelMA bioink has never been quicker!

Applications

In a low concentration formulation for use with the BIO X EMD Printhead to generate microspheres
In a high concentration formulation to generate bioinks such as GelMA Bioink or blended with other additives to generate your own unique bioink.
Compatible with thickeners such as Nanofibrillated Cellulose, Xanthan Gum, and Glucomannan.
After 3D cell culture you can easily harvest the cells for analysis using the Cell Collect G Bioink Lysis Reagent.
Recommended for use with BIO X, a temperature-controlled printhead and a heated printbed. Not recommended for use with INKREDIBLE+ or INKREDIBLE.

Standard Bioink

GelMA HA

GelMA HA incorpora acido ialuronico metacrilato nel nostro bioink GelMA. L'acido ialuronico è vitale per un'ampia varietà di tessuti diversi tra cui pelle, cartilagine, ossa, tessuto connettivo e nervi. Pronto da stampare fuori dalla scatola, potete usarlo per fabbricare rapidamente costrutti per molte applicazioni. Disponibile con LAP come fotoiniziatore allo 0,25%.

Sei interessato a sviluppare la tua formulazione di bioink? Dai un'occhiata ai nostri GelMA e HAMA Kit per personalizzare il tuo bioink.

Raccomandato per l'uso con BIO X, una testina di stampa a temperatura controllata e un piano di stampa riscaldato. Non consigliato per l'uso con INKREDIBLE+ o INKREDIBLE.

Standard Bioink

GelMA Fibrin

Il bioink GelMA FIBRIN è progettato per fornire segnali biologici e meccanici rilevanti per le cellule endoteliali che sono responsabili della formazione dei vasi sanguigni. Per renderlo possibile, abbiamo aggiunto a GelMA una funzionalità specifica per il tessuto endoteliale. Con l'aiuto del fibrinogeno, una glicoproteina che può essere convertita enzimaticamente in fibrina, miriamo a mediare la diffusione delle cellule endoteliali e la proliferazione dei fibroblasti per imitare il processo fisiologico di vascolarizzazione dei tessuti all'interno dei costrutti GelMA bioprintati.

Raccomandato per l'uso con la testina di stampa a temperatura controllata BIO X e il piano di stampa raffreddato, o per l'uso con INKREDIBLE+ e la testina di stampa riscaldata.

Non raccomandato      per l'uso con INKREDIBLE.

Standard Bioink

GelMA C

GelMA C combines the advantages of GelMA and nanofibrillated cellulose. This blend offers smooth printability at room temperature without temperature control, and provides fibrillar morphology to benefit specific cell types. GelMA C crosslinks quickly through photocuring and with our ionic crosslinking solution. The ink retains its shear-thinning properties in physiological conditions and provides excellent printability at low extrusion pressures using a wide range of nozzle diameters. Available with LAP photoinitiator at 0.25 percent.

Interested in making your making your own blend? Check out our GelMA Kit and Nanofibrillated Cellulose to tailor your own composition.

Recommended for use with BIO X, a temperature-controlled printhead and a cooled printbed, or INKREDIBLE+ with a heated printhead. Not recommended for use with INKREDIBLE.

Standard Bioink

Gelma A

GelMA A is a blend of GelMA and alginate, offering a larger printability window compared to pure GelMA bioinks due to the softening provided by the alginate. GelMA A can be stabilized through photocrosslinking, or through the addition of the ionic crosslinking solution. GelMA A is a shear-thinning bioink that prints at low pressures and forms filaments once deposited. Available with LAP photoinitiator at 0.25 percent.

Check out our GelMA-Alginate Kit if you want to make your own formulations.

Advanced formulation available that contains Xanthan Gum for better printability in our GelXA bioink series.

Recommended for use with BIO X, a temperature-controlled printhead and a cooled printbed, or INKREDIBLE+ with a heated printhead. Not recommended for use with INKREDIBLE.

Standard Bioink

Chitoink

Chitoink is the first ever commercialized chitosan-based bioink for 3D bioprinting. The cationic polysaccharide chitosan is derived from the naturally occurring biomaterial chitin. Chitosan forms mechanically stable hydrogels with good cell affinity and has been extensively used in research related to tissue engineering of cartilage, skin and bone. By incorporating glucomannan and a glycerol phosphate salt, Chitoink provides good printability at physiological pH, high shape fidelity, sufficient mechanical properties and biocompatibility. After bioprinting, Chitoink crosslinks easily with the included ionic TPP Crosslinking Agent.

Standard Bioink

Cellink Skin

CELLINK SKIN has fibrinogen to recreate the natural wound-healing environment found in skin tissue. It is optimized for culturing fibroblasts and keratinocytes to fabricate skin constructs. CELLINK SKIN comes with an enhanced thrombin-containing crosslinking solution to develop a compound network with unparalleled stability. After crosslinking with thrombin, CELLINK SKIN’s in situ fibrin and fibrinogen provides an environment that mimics the natural wound-healing process.

For a complete solution (including post analysis antibodies) check out our Skin Tissue Model Kit

 

Below you can download a protocol for Bioprinting CELLINK SKIN as well as printing a Skin Tissue Model with the G-code found on Bioverse.

Standard Bioink

Cellink RGD

Basato su CELLINK Bioink, il bioink CELLINK RGD fornisce eccellenti proprietà di stampabilità e un ambiente cellulare naturale con aggiunta di biofunzionalizzazione RGD per migliorare il legame cellulare. Usa il nostro CELLMIXER per miscelare CELLINK RGD con un'alta concentrazione di cellule e consentire un processo di bioprinting in una sola fase. Dopo il bioprinting, CELLINK RGD si lega facilmente con la soluzione ionica inclusa.

Standard Bioink

Cellink Laminink +

Laminins are proteins composed of three subunits — α-chain, β-chain and γ-chain. They are a major component of the basal lamina lining a cell membrane’s external surface. Its cross-like structure serves as the foundation for many tissues. Laminins play an important role in cell differentiation, migration, adhesion and the support of healthy tissue.

CELLINK LAMININK+ is an enhanced formulation of our CELLINK Bioink that contains several laminins and offers excellent printability and easy crosslinking, and is a great starting point for culturing many cell types in 3D. Also available with a GelXA base.

Uses

  • Use as a throughput 3D model (deposited as droplets) with the BIO X Temperature Controlled or Syringe Printhead.
  • As a bioprinted 3D construct as demonstrated in the Liver Tissue Model Kit

Other specialized cell specific formulations available:

  • CELLINK LAMININK 111
  • CELLINK LAMININK 121
  • CELLINK LAMININK 411
  • CELLINK LAMININK 521

Standard Bioink

Cellink Laminink 411

Laminins are proteins composed of three subunits — α-chain, β-chain and γ-chain. They are a major component of the basal lamina lining a cell membrane’s external surface. Its cross-like structure serves as the foundation for many tissues. Laminins play an important role in cell differentiation, migration, adhesion and the support of healthy tissue.

CELLINK LAMININK 411 is an enhanced formulation of our CELLINK Bioink that supports different tissues types, including tissues from the pancreas, vascular, immune, nervous and hematopoietic systems. This bioink is a suitable base material for culturing specific cell types, including pancreatic cells and neurons. You can use the bioink for developing pancreas models and studying brain and nerve tissues. Also available with a GelXA base.

Uses

  • Use as a throughput 3D model (deposited as droplets) with the BIO X Temperature Controlled or Syringe Printhead.
  • As a bioprinted 3D construct as demonstrated in the Liver Tissue Model Kit

Other specialized cell specific formulations available:

  • CELLINK LAMININK 111
  • CELLINK LAMININK 121
  • CELLINK LAMININK 521
  • CELLINK LAMININK+

Standard Bioink

Cellink Laminink 121

Laminins are proteins composed of three subunits — α-chain, β-chain and γ-chain. They are a major component of the basal lamina lining a cell membrane’s external surface. Its cross-like structure serves as the foundation for many tissues. Laminins play an important role in cell differentiation, migration, adhesion and the support of healthy tissue.

CELLINK LAMININK 121 is an enhanced formulation of our CELLINK Bioink that can be used as the starting point for culture of multiple cell types. Laminin 121 is expressed widely during embryonic and early development stages of organ and tissue lining. This laminin variation is mostly found in placenta, skeletal muscle and kidney tissue. CELLINK LAMININK 121 is particularly suitable as a base material for culturing cell types including skeletal muscle, brain, kidney and liver cells. Also available with a GelXA base.

Uses

  • Use as a throughput 3D model (deposited as droplets) with the BIO X Temperature Controlled or Syringe Printhead.
  • As a bioprinted 3D construct as demonstrated in the Liver Tissue Model Kit

Other specialized cell specific formulations available:

  • CELLINK LAMININK 111
  • CELLINK LAMININK 411
  • CELLINK LAMININK 521
  • CELLINK LAMININK+

Standard Bioink

Cellink Laminink 111

Laminins are proteins composed of three subunits referred to as a α-chain, β-chain and γ-chain. They are a major component of the basal lamina that lines the external surface of cell membranes. Its cross-like structure that serves as a foundation for many tissues. Laminins play an important role in cell differentiation, migration, adhesion and also involved in supporting healthy tissue.

CELLINK LAMININK 111 is an enhanced formulation of our CELLINK Bioink that contains Laminin 111 which is an important protein during early development of the lining of tissues and organs. Specialized applications that this bioink is suited for include the culture of brain, kidney, liver or intestinal cells. In disease modeling, this bioink is relevant for studying Parkinson’s disease and cancer models and is a great starting point for culturing many cell types. Also available with a GelXA base.

Uses

  • Use as a throughput 3D model (deposited as droplets) with the BIO X Temperature Controlled or Syringe Printhead.
  • As a bioprinted 3D construct as demonstrated in the Liver Tissue Model Kit

Other specialized cell specific formulations available:

  • CELLINK LAMININK 121
  • CELLINK LAMININK 411
  • CELLINK LAMININK 521
  • CELLINK LAMININK+

Standard Bioink

Cellink Gelma

CELLINK gelatin methacrylate (GelMA) is a gelatin-based bioink that provides mammalian cells with the essential properties of their native environment. GelMA is modified with methacryloyl groups to crosslink with a photoinitiator (like LAP) or exposure to UV light (available using INKREDIBLE+, BIO X, and a 365 nm or 405 nm photocuring system). Available with a 0.25 percent LAP photoinitiator.

Available as lyophilized powder in our GelMA Kit for tailoring your own bioink formulation

Advanced formulations based off of CELLINK GelMA

  • GelMA A
  • GelMA C
  • GelMA HA
  • GelXA
  • GelXG

Standard Bioink

Cellink Bone

Based on the CELLINK Bioink, CELLINK BONE bioink offers the same printability and natural 3D environment as CELLINK Bioink with added biofunctionalization. This bioink is composed of tricalcium phosphate osteoconductive particles to enhance bone tissue engineering applications. Use our CELLMIXER to mix CELLINK BONE with a high concentration of cells and enable a one-step bioprinting process. After bioprinting, CELLINK BONE crosslinks easily with the included ionic crosslinking agent.

Also available with a GelXA base.

Recommended to use the CELLMIXER for blending in cells.

Uses

  • Use as a  3D model (example use here) with the BIO X Temperature Controlled or Syringe Printhead.
  • Printed in conjugation with the BIO X Thermoplastic Printhead to complement a thermoplastic scaffold such as PCL.

 

Standard Bioink

CELLINK BIOINK

CELLINK Bioink is the first universal bioink designed to print human tissue models with any 3D-bioprinting system, commercially available or developed in-house. CELLINK Bioink is a non-animal derived polysaccharide hydrogel that is ideal for 3D bioprinting and cell culturing. CELLINK Bioink is composed of alginate and highly hydrated cellulose nanofibrils with morphological similarity to collagen, providing mammalian cells with a milieu that resembles their natural matrix. Its excellent printability enables you to bioprint complex, cell-laden tissue constructs, such as a human auricle. The unique biocompatibility and printability of CELLINK Bioink offer outstanding results that will take your research to the next level! Use our CELLMIXER to mix CELLINK Bioink with a high concentration of cells and  a one-step bioprinting process. After bioprinting, CELLINK Bioink crosslinks easily with the included ionic crosslinking agent.

Specialized Formulations Based off the CELLINK Bioink

  • CELLINK RGD
  • CELLINK FIBRIN
  • CELLINK SKIN
  • CELLINK BONE
  • CELLINK LAMININK

Standard Bioink

BIO CONDUCTINK

Bio Conductink is an advanced bioink derived from our GelMA SERIES of bioinks. This formulation is electrically conductive intended for use in muscular contraction and neural tissue models. Contains LAP as photoinitiator at 0.25 percent.

The printability of this bioink is temperature sensitive and therefore is recommended for use with the BIO X Temperature-controlled Printhead or the INKREDIBLE+ bioprinter system. Not recommended for use with the INKREDIBLE system


© COPYRIGHT 2020 TWIN HELIX C.F. /P.IVA and n° Registro Imprese di Milano 05819650960 – REA Milano 1851394 Cap.Soc.Euro 50.000 i.v. - Società uninominale